Genomic PCR Grade Proteinase K Powder

Availability

Please contact us to check the latest availability and to check prices. For larger or bulk quantity a discount may apply
CAS No

39450-01-6
Application

nonspecific serine protease
Ordering

Product made-to-order. Please contact us for latest inventory stock. Delivery is 1-2 weeks upon order confirmation
Package weight

The standard package weight with individual packaging is 0,05
Before use

Store at 2-8°C
Shipped to you

Shipped at room temperature
Technical file

Please inquire for the latest datasheet, certificate of analysis and SDS
Precautions

For Research Use Only. Manufactured in USA
Other products
Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Gene

Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).

Gene target

Genomic Grade Proteinase Powder
Short name

Genomic PCR Grade Proteinase K Powder
Technique

PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Genomic PCR test kit Grade Proteinase K Powder
Alternative technique

dna-amplification

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data