HSP60 ELISA Kit

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• Catalog number
  SKT-110-480
• Price:

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• Size
  5 x 96 well

# #

• Stock availability
  In Stock
• Kit s description
  Colorimetric detection of HSP60
• Protein target
  HSP60
• Scientific context
  In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP,iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimers, coronary artery diseases, MS, and diabetes (6-9).
• Primary research area
  Cancer, Heat Shock
• Category
  Assay ELISA Kits
• Other name
  CPN60 ELISA Kit, GROEL ELISA Kit, HLD4 ELISA Kit, HSP 60 ELISA Kit, HSP65 ELISA Kit, HSPD1 ELISA Kit, HuCHA60 ELISA Kit, SPG 13 ELISA Kit
• Brand name
  StressXpress®
• Detection system
  Colorimetric Assay
• Assay format
  Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
• Main intended usage
  ELISA kit used to quantitate HSP60 concentration in samples.
• Brief protocol
  1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at 37°C for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
• Kit contents
  Anti-Hsp60 Immunoassay Plate, 5X Hsp60 Extraction Reagent, Recombinant Hsp60 Standard, Standard and Sample Diluent, 10X Wash Buffer Concentrate, Anti-Hsp60 Biotinylated Antibody Concentrate, Anti-Hsp60 Biotinylated Antibody Diluent, Streptavidin: HRP Concentrate, Streptavidin: HRP Diluent, TMB Substrate, Stop Solution
• Assay precision
  Contact us
• Assay cross reactivity
  Human
• Samples to be used with this kit
  Cell Lysates, Tissue, Serum
• Maximum samples to be used with this kit
  40 samples in duplicate
• Assay duration
  30 minutes
• Kit s sensitivity
  1,37 ng/ml
• Assay detection limit
  7 - 450 ng/ml
• Storage recommendations
  4°C
• Shipping recommendations
  Blue Ice
• Bibliography
  1. Hartl F.U. (1996) Nature. 381: 571-579. 2. Bukau B. and Horwich, A.L. (1998) Cell. 92: 351-366. 3. Hartl F.U. and Hayer-Hartl, M. (2002) Science. 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biology. 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta S. and Knowlton A.A. J. Cell Mol. Med. 9: 51-58. 8. Deocaris C.C., et al. (2006) Cell Stress Chaperones. 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.
• Release date
  1-Jun-2011
• PubMed number
  Refer to PubMed
• Tested applications
  No data available / currently testing
• Tested reactivity
  No data available / currently testing
• Resources available upon request
  Kit Booklet, MSDS
• Product image link
• Product image legend
  Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 7 - 450 ng/mL. Graph of the Typical Standard Curve for the HSP60 ELISA Kit StressXpress - SKT-110
• Warnings
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  2
• Net weight g
  2500
• Other related products
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

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• Gene target
  HSP60  
• Gene symbol
  HSPD1
• Short name
  HSP60 ELISA Kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Alternative name
  HSP60 Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:5261
• Gene
  HSPD1
• Long gene name
  heat shock protein family D (Hsp60) member 1
• Synonyms gene
  • SPG13
• Synonyms gene name
  • heat shock 60kD protein 1 (chaperonin)
  • spastic paraplegia 13 (autosomal dominant)
  • heat shock 60kDa protein 1 (chaperonin)
• Synonyms
  • GroEL
  • HSP60
• GenBank acession
  • M34664
• Locus
  2q33.1
• Discovery year
  1991-07-19
• Entrez gene record
  3329
• Pubmed identfication
  • 1980192
  • 11898127
• RefSeq identity
  • NM_002156
• Classification
  • Chaperonins
• VEGA ID
  OTTHUMG00000154463

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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