Mouse Anti-Human HSP70 Monoclonal IgG, Clone C92F3A-5

#

• Catalog number
  SMC-100B-PCP
• Price:

  Please ask

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• Size
  200 µg

# #

• Clone
  C92F3A-5
• Immunogen
  Human HSP70
• Antibody s full description
  Mouse Anti-Human HSP70 Monoclonal IgG Antibody, Clone: C92F3A-5: PerCP
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
• Raised in
  Mouse
• Antibody s target
  HSP70
• Primary research fields
  Cancer, Heat Shock
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF, ELISA, FCM, FACS, IEM, Bl, AM
• Antibody s reactivity
  Human, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Dog, Chicken, Fruit Fly (Drosophila melanogaster), Carp (Cypriniformes), Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep
• Antibody s dilutions
  WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cytoplasm
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
• Bibliography
  1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. 8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. 10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. 11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. 12. Dressel et al. (2000) J. Immunol. 164: 2362-2371. 13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. 14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
• Released date
  1-Jan-2007
• PubMed number
  28052117, 28053100, 27524398, 27509516, 27527204, 27409630, 27354066, 25552722, 25552722, 25355627, 25412999, 25412999, 25412999, 25034784, 25356750, 25356750, 25200073, 24903326, 25038226, 25400510, 25024384, 24705179, 24625088, 24447452, 24447452, 24516376, 23429211, 23598404, 22763123, 22343013, 22496374, 22050377, 21975426, 22010006, 22039833, 22039833, 21737627, 21320889, 21522149, 21522149, 20924639, 21253609, 21253609, 20130532, 19706692, 19056281, 19209902
• Tested applications
  Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Flow Cytometry, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Western Blot, Immunoprecipitation, Flow Cytometry, Western Blot, Western Blot, Protein Inhibition, Western Blot, Western Blot, Immunoprecipitation, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Electron Microscopy, Immunoprecipitation , Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot
• Tested reactivity
  Human, Human, Mouse, Human, Human, Human, Human, Human, Human, Human, Bovine, Bovine, Bovine, Human, Mouse, Mouse, Human, Mouse, Mouse, Rat, Human, Rat, Mouse, Human, Human, Mouse, Pig, Human, Rat, Human, Dog, Human , Rat, Pig, Human, Human, Human, Rat, Human, Human, Human, Mouse, Human, Human, Pig, Mouse, Rat
• NCBI number
  NP_005336.3
• Gene number
  3303
• Protein number
  P08107
• Antibody s datasheet
  Contact our support service
• Representative figure link
  details | see here | read more | look at | more details
• Representative figure legend
  Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (SMC-100) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida. Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Mouse colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Heat Shocked Melanoma cells (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Western Blot (WB) on Human cell lysates from various cell lines (SMC-100) Mouse Anti-Hsp70 Antibody: FITC [C92] used in Fluorescence Activated Cell Sorting (FACS) on Mouse Heat Shocked CD3+ CD8+ T cells (SMC-100)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.2
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Latin name
  Mus musculus

#

• Gene target
  HSP70   Monoclonal   C92F3A-5  
• Gene symbol
  HSPA1B, HSPA14, HSPA1A, HSPA1L, MIR1302-5, PIRC23, PIRC22, PIRC20, PIRC21, HTR6
• Short name
  Mouse Anti- HSP70 Monoclonal IgG, Clone C92F3A-5
• Technique
  anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG, IgG
• Label
  PerCP
• Species
  Human, Humans, Mouses
• Alternative name
  Mouse Antibody toHuman HSP70 monoclonal Immunoglobulin G, clonality C92F3A-5
• Alternative technique
  murine, antibodies, immunoglobulins
• Clone name
  C92F3A-5

Gene info

• Identity
  HGNC:5233
• Gene
  HSPA1B
• Long gene name
  heat shock protein family A (Hsp70) member 1B
• Synonyms gene name
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
• Synonyms
  • HSP70-2
• Locus
  6p21.33
• Discovery year
  1995-08-11
• Entrez gene record
  3304
• Pubmed identfication
  • 1700760
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031202

Gene info

• Identity
  HGNC:29526
• Gene
  HSPA14
• Long gene name
  heat shock protein family A (Hsp70) member 14
• Synonyms gene name
  • heat shock 70kDa protein 14
• Synonyms
  • HSP70-4
  • HSP70L1
• GenBank acession
  • AF112210
• Locus
  10p13
• Discovery year
  2004-04-20
• Entrez gene record
  51182
• Pubmed identfication
  • 21208456
• RefSeq identity
  • NM_016299
• Classification
  • Heat shock 70kDa proteins

Gene info

• Identity
  HGNC:5232
• Gene
  HSPA1A
• Long gene name
  heat shock protein family A (Hsp70) member 1A
• Synonyms gene
  • HSPA1
• Synonyms gene name
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
• Synonyms
  • HSP70-1
• GenBank acession
  • BC002453
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  3303
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031201

Gene info

• Identity
  HGNC:5234
• Gene
  HSPA1L
• Long gene name
  heat shock protein family A (Hsp70) member 1 like
• Synonyms gene name
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
• Synonyms
  • HSP70-HOM
  • hum70t
• GenBank acession
  • D85730
• Locus
  6p21.33
• Discovery year
  1989-05-12
• Entrez gene record
  3305
• Pubmed identfication
  • 9685725
  • 9349405
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031208

Gene info

• Identity
  HGNC:35297
• Gene
  MIR1302-5
• Long gene name
  microRNA 1302-5
• Synonyms gene
  • MIRN1302-5
• Synonyms
  • hsa-mir-1302-5
• Locus
  20q13.13
• Discovery year
  2008-11-03
• Entrez gene record
  100302146
• RefSeq identity
  • NR_031634
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37527
• Gene
  PIRC23
• Long gene name
  piwi-interacting RNA cluster 23
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313786
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37526
• Gene
  PIRC22
• Long gene name
  piwi-interacting RNA cluster 22
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313850
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37524
• Gene
  PIRC20
• Long gene name
  piwi-interacting RNA cluster 20
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313785
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37525
• Gene
  PIRC21
• Long gene name
  piwi-interacting RNA cluster 21
• Locus
  5
• Discovery year
  2009-11-05
• Entrez gene record
  100313916
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:5301
• Gene
  HTR6
• Long gene name
  5-hydroxytryptamine receptor 6
• Synonyms gene name
  • 5-hydroxytryptamine (serotonin) receptor 6
  • 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
• Synonyms
  • 5-HT6
  • 5-HT6R
• GenBank acession
  • L41147
• Locus
  1p36.13
• Discovery year
  1997-12-11
• Entrez gene record
  3362
• Pubmed identfication
  • 8522988
• RefSeq identity
  • NM_000871
• Classification
  • 5-hydroxytryptamine receptors, G protein-coupled
• VEGA ID
  OTTHUMG00000002713

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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