PCR kit (Reverse transcription and PCR) with Electrophoretic Detection, HCV - 240/440 IC (RT-PCR) PCR Test for detection of HCV

• Catalog number
  V-1-100R
• Price:

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• Size
  110 tests

• Description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
• Gene
  Hepatitis C virus (HCV) NS3, NS4, NS genotypes 1, 2, 3, 4, 5, are small (55–65 nm in size), enveloped, positive-sense single-stranded RNA viruses. Hepatitis C virus is the cause of hepatitis C and some cancers such as Liver Cancer (Hepatocellular carcinoma abbreviated HCC) and lymphomas in humans. HCV Core antigens or envelope proteins are used for immunization to obtain diagnostic antibodies.
• Group
  PCR, polymerase chain reaction, RT-PCR mixes
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Goup
  reverse transcription

• Gene target
  kit   transcription   with   Electrophoretic   HCV   240/440   Test   for   HCV  
• Short name
  PCR kit (Reverse transcription PCR) with Electrophoretic , HCV - 240/440 IC (RT-PCR) PCR Test for of HCV
• Technique
  detection, reverse, RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  PCR test kit reagent (Reverse transcription and PCR test kit) including Electrophoretic quantification, Hepatitis C virus - 240/440 IC (Reverse transcription PCR test kit) PCR test kit Test to measure quantification on Hepatitis C virus
• Alternative technique
  transctription, rtpcrkits, tests, kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Reverse Transcriptase Polymerase Chain Reaction
• Concept
  Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
• Tree numbers
  • E05.393.620.500.725
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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