Escherichia coli Histidine--tRNA ligase (hisS) -Baculovirus

Long name

Recombinant Escherichia coli Histidine--tRNA ligase (hisS)
Alternative names

histidyl-tRNA synthetase; Histidine--tRNA ligase; histidyl-tRNA synthetase; Histidyl-tRNA synthetase
Gene name

hisS
Other gene names

hisS; hisS; HisRS
General description

Histidine--tRNA ligase (hisS) is a recombinant protein expressed in Baculovirus . The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
Product category

Recombinant Proteins
Expression system

Baculovirus
Available also expressed in

E Coli ; Yeast ; Baculovirus ; Mammalian Cell
Purity

Greater than 90% (determined by SDS-PAGE)
Form

Lyophilized protein
Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data