Human KRT10(Keratin, Type I Cytoskeletal 10) EIA Kit

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• Catalog number
  E-EL-H2118-96T
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• Size
  96Test

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• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  CK10, K10, Cytokeratin-10, Keratin-10, EHK, KPP, BCIE, BIE
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRT10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRT10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KRT10. You can calculate the concentration of Human KRT10 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  9.38pg/mL
• Detection Range
  15.63~1000pg/mL
• Target Name
  KRT10
• UNIProt ID
  P13645
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human KRT10(Keratin, Type I Cytoskeletal 10) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

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• Gene target
  KRT10   Keratin   Cytoskeletal   Kit  
• Gene symbol
  KRT10, KRT10-AS1
• Short name
  KRT10(Keratin, I Cytoskeletal 10) EIA Kit
• Technique
  EIA, eia
• Species
  Human, Humans
• Alternative name
  H. sapiens KRT10(Keratin, classification I Cytoskeletal 10) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:6413
• Gene
  KRT10
• Long gene name
  keratin 10
• Synonyms gene
  • KPP
• Synonyms gene name
  • keratosis palmaris et plantaris
  • keratin 10, type I
• Synonyms
  • K10
  • CK10
• Synonyms name
  • cytokeratin 10
  • epidermolytic hyperkeratosis
• GenBank acession
  • J04029
• Locus
  17q21.2
• Discovery year
  1988-08-12
• Entrez gene record
  3858
• Pubmed identfication
  • 2461420
  • 16831889
• RefSeq identity
  • NM_000421
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133368

Gene info

• Identity
  HGNC:28305
• Gene
  KRT10-AS1
• Long gene name
  KRT10 antisense RNA 1
• Synonyms gene
  • TMEM99
• Synonyms gene name
  • transmembrane protein 99
  • transmembrane protein 99 (putative)
• Synonyms
  • MGC21518
• GenBank acession
  • AK097454
• Locus
  17q21.2
• Discovery year
  2005-12-16
• Entrez gene record
  147184
• Pubmed identfication
  • 29561261
• RefSeq identity
  • NR_160886
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000133579

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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