AGS Cell Line

#

• Catalog number
  EP-CL-0022
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• Size
  1×10⁶ cells/vial

# #

• Abbreviation
  AGS
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  54 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection . Subsequently, AGS has been determined to be infected with Parainfluenza type 5(PIV5 formerly known as SV5).
• Biosafety
  2
• Medium
  Ham's F-12 (PM150810)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:6
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in athymic BALB/c mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

#

• Gene target
  AGS   Line  
• Gene symbol
  JAG1
• Short name
  AGS Line
• Species
  Homo sapiens, Human
• Alternative name
  AGS cellular Line
• Tissue
  Stomach

Gene info

• Identity
  HGNC:6188
• Gene
  JAG1
• Long gene name
  jagged canonical Notch ligand 1
• Synonyms gene
  • AGS
  • JAGL1
• Synonyms gene name
  • Alagille syndrome
  • jagged 1
• Synonyms
  • AHD
  • AWS
  • HJ1
  • CD339
• GenBank acession
  • U61276
• Locus
  20p12.2
• Discovery year
  1997-05-22
• Entrez gene record
  182
• Pubmed identfication
  • 7697721
  • 9207788
• RefSeq identity
  • NM_000214
• Classification
  • CD molecules
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000031872
• Locus Specific Databases
  • JAG1 database at LOVD-China
  • LRG_1191

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