Oxidosqualene Cyclase (OSC) Polyclonal Antibody (Rat), FITC

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• Catalog number
  PAB898Ra01-100ul-FITC
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• Size
  100ul

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• Description
  A Rabbit polyclonal antibody against Rat Oxidosqualene Cyclase (OSC). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: OSC (Gly101~Ser250); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• French translation
  anticorps

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• Gene target
  Oxidosqualene   Cyclase   OSC  
• Gene symbol
  LSS
• Short name
  Oxidosqualene Cyclase (OSC) Polyclonal Antibody ( ), FITC
• Technique
  Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rat
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  Oxidosqualene Cyclase (OSC) polyclonal (antibody to-) (Rat), fluorecein
• Alternative technique
  polyclonals, antibodies, fluorescine

Gene info

• Identity
  HGNC:6708
• Gene
  LSS
• Long gene name
  lanosterol synthase
• Synonyms gene name
  • lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
• Synonyms
  • OSC
• Synonyms name
  • Oxidosqualene-lanosterol cyclase
• GenBank acession
  • U22526
• Locus
  21q22.3
• Discovery year
  1998-05-07
• Entrez gene record
  4047
• Pubmed identfication
  • 7639730
  • 8655142
• VEGA ID
  OTTHUMG00000090633

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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