High Mobility Group AT Hook Protein 2 (HMGA2) Polyclonal Antibody (Mouse), FITC

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• Catalog number
  PAC053Mu01-100ul-FITC
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• Size
  100ul

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• Description
  A Rabbit polyclonal antibody against Mouse High Mobility Group AT Hook Protein 2 (HMGA2). This antibody is labeled with FITC.
• Specifications
  Host: Rabbit; Species Reactivity: Mouse; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
• Additional_information
  Sequence of the immunogen: HMGA2 (Met1~Asp108); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Storage_and_shipping
  Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
• Notes
  Research Use Only.
• Properties
  If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

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• Gene target
  Mobility   Group   Hook   Protein   HMGA2  
• Gene symbol
  HMGA2, HMGA2-AS1
• Short name
  Mobility Group AT Hook Protein 2 (HMGA2) Polyclonal Antibody (Mouse), FITC
• Technique
  Polyclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  mouse
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  High Mobility family AT Hook Protein 2 (HMGA2) polyclonal (antibody to-) (Mouse), fluorecein
• Alternative technique
  polyclonals, antibodies, murine, fluorescine

Gene info

• Identity
  HGNC:5009
• Gene
  HMGA2
• Long gene name
  high mobility group AT-hook 2
• Synonyms gene
  • HMGIC
• Synonyms gene name
  • high-mobility group (nonhistone chromosomal) protein isoform I-C
• Synonyms
  • BABL
  • LIPO
• GenBank acession
  • U28754
• Locus
  12q14.3
• Discovery year
  1998-10-23
• Entrez gene record
  8091
• Pubmed identfication
  • 8824803
  • 9003504
• RefSeq identity
  • NM_003483
• Classification
  • Canonical high mobility group
• VEGA ID
  OTTHUMG00000168936

Gene info

• Identity
  HGNC:53973
• Gene
  HMGA2-AS1
• Long gene name
  HMGA2 antisense RNA 1
• Synonyms
  • RP11-366L20.2
• Locus
  12q14.3
• Discovery year
  2018-10-10
• Entrez gene record
  100129940
• RefSeq identity
  • NR_120478
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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