Anti-PP2A alpha + beta (Polyclonal), ALEXA Fluor 594

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• Catalog number
  GENTObs-0029R-A594
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• Size
  100 microliters

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• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  PP2A alpha + beta
• Specificity
  This antibody reacts specifically with PP2A alpha + beta
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm, Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from human PP-2A
• Gene ID
  5515
• Swiss Prot
  P67775
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV4 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  RP-C; PP2Ac; PP2CA; PP2Calpha; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; PPP2CA
• Also known as
  PP2A alpha + beta Polyclonal Antibody
• Other name
  Anti-PP2A alpha + beta Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  The Anti-PP2A alpha + beta is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

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• Gene target
  PP2A   alpha   beta  
• Gene symbol
  PPP2R1B, PPP2R1A
• Short name
  Anti-PP2A alpha + beta
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  PP2A alpha + beta Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:9303
• Gene
  PPP2R1B
• Long gene name
  protein phosphatase 2 scaffold subunit Abeta
• Synonyms gene name
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform
  • protein phosphatase 2 regulatory subunit A, beta
• Synonyms
  • PR65B
  • PP2A-Abeta
• Synonyms name
  • PP2A-A-beta
  • protein phosphatase 2A, regulatory subunit A, beta isoform
• GenBank acession
  • AF087438
• Locus
  11q23.1
• Discovery year
  1993-01-25
• Entrez gene record
  5519
• Pubmed identfication
  • 2159327
  • 9795170
• RefSeq identity
  • NM_002716
• Classification
  • STRIPAK complex
  • Protein phosphatase 2 scaffold subunits
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000166741

Gene info

• Identity
  HGNC:9302
• Gene
  PPP2R1A
• Long gene name
  protein phosphatase 2 scaffold subunit Aalpha
• Synonyms gene name
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform
• Synonyms
  • PR65A
  • PP2A-Aalpha
  • PP2AA
• Synonyms name
  • protein phosphatase 2A, regulatory subunit A, alpha isoform
  • protein phosphatase 2, 65kDa regulatory subunit A
  • protein phosphatase 2A structural subunit A, alpha isoform
• Locus
  19q13.41
• Discovery year
  1993-01-25
• Entrez gene record
  5518
• RefSeq identity
  • NM_014225
• Classification
  • STRIPAK complex
  • Protein phosphatase 2 scaffold subunits
  • Armadillo like helical domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000137367

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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