17-Hydroxyprogesterone (17-OHP) ELISA Kit, 96 well plate

#

• Catalog number
  EKU02011
• Price:

  Please ask

  Ask for price
• Size
  5x96T

# #

• Verified reactivity
  Multispecies (General)
• Detection limits
  246.9-20000 pg/mL
• Sensitivity limit
  87,3 pg/mL
• Samples to be analyzed
  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay duration
  2 hours
• Verified applications
  ELISA
• Assay class
  Competitive
• ELISA detection
  Colorimetric
• Protein number
  Please refer to Uniprot
• Alternate protein number
  Please refer to SwissProt
• Gene number
  Please refer to GenBank
• Gene name
  17-Hydroxyprogesterone
• Alternate gene name
  17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
• Estimated production time
  7-11 business days
• Shipping requirements
  Blue ice
• Storage recommendation
  -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
• Use for
  8 months
• ELISA s specificity
  This assay has high sensitivity and excellent specificity for detection of 17-Hydroxyprogesterone (17-OHP). No significant cross-reactivity or interference between 17-Hydroxyprogesterone (17-OHP) and analogues was observed.
• ELISA s cross reactivity
  This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
• Assay principle
  This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 17-Hydroxyprogesterone (17-OHP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 17-Hydroxyprogesterone (17-OHP) and unlabeled 17-Hydroxyprogesterone (17-OHP) (Standards or samples) with the pre-coated antibody specific to 17-Hydroxyprogesterone (17-OHP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 17-Hydroxyprogesterone (17-OHP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 17-Hydroxyprogesterone (17-OHP) in the sample.
• Protocol
  Please see ELISA's datasheet, otherwise contact us
• Precision of the test
  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Hydroxyprogesterone (17-OHP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Hydroxyprogesterone (17-OHP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
• ELISA s stability
  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
• QC
  The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
• Research main area
  Endocrinology;Reproductive science;Hormone metabolism;
• Warnings
  The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
• Description
  A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

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• Gene target
  17-Hydroxyprogesterone   17-OHP  
• Gene symbol
  PIRC95, PIRC91, PIRC90, PIRC93, PIRC92, SNORD115-17, IGHV3OR16-17, SNORD116-17
• Short name
  17-Hydroxyprogesterone (17-OHP) ELISA Kit, 96
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Alternative name
  17-Hydroxyprogesterone (17-OHP) Enzyme-linked immunosorbent assay reagent, 96 well plate
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:37599
• Gene
  PIRC95
• Long gene name
  piwi-interacting RNA cluster 95
• Locus
  17
• Discovery year
  2009-11-05
• Entrez gene record
  100313935
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37595
• Gene
  PIRC91
• Long gene name
  piwi-interacting RNA cluster 91
• Locus
  17
• Discovery year
  2009-11-05
• Entrez gene record
  100313900
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37594
• Gene
  PIRC90
• Long gene name
  piwi-interacting RNA cluster 90
• Locus
  17
• Discovery year
  2009-11-05
• Entrez gene record
  100313798
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37597
• Gene
  PIRC93
• Long gene name
  piwi-interacting RNA cluster 93
• Locus
  17
• Discovery year
  2009-11-05
• Entrez gene record
  100313934
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37596
• Gene
  PIRC92
• Long gene name
  piwi-interacting RNA cluster 92
• Locus
  17
• Discovery year
  2009-11-05
• Entrez gene record
  100313920
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:33036
• Gene
  SNORD115-17
• Long gene name
  small nucleolar RNA, C/D box 115-17
• Synonyms
  • HBII-52-17
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033455
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003309.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:55105
• Gene
  IGHV3OR16-17
• Long gene name
  immunoglobulin heavy variable 3/OR16-17 (non-functional)
• Synonyms
  • IGHV3/OR16-17
• Locus
  16p11.2
• Discovery year
  2020-06-26
• Entrez gene record
  390714
• Classification
  • Immunoglobulin heavy (IGH) orphons

Gene info

• Identity
  HGNC:33083
• Gene
  SNORD116-17
• Long gene name
  small nucleolar RNA, C/D box 116-17
• Synonyms
  • HBII-85-17
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033429
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003332
• Classification
  • Small nucleolar RNAs, C/D box

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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